MORPHOLOGICAL AND FUNCTIONAL STATE OF HUMAN NEUTROPHILS UNDER THE INFLUENCE OF FEMTOSECOND LASER PULSES IN VITRO 

Gening Tatyana Petrovna, Doctor of biological sciences, professor, head of sub-department of physiology and pathophysiology, Ulyanovsk State University, Naum-53@yandex.ru
Abakumova Tatyana Vladimirovna, Candidate of biological sciences, senior staff scientist, laboratory of molecular and cell biology, Research Technological Institute, Ulyanovsk State University, Naum-53@yandex.ru
Arslanova Dinara Rishatovna, Candidate of biological sciences, senior staff scientist, laboratory of molecular and cell biology, Research Technological Institute, Ulyanovsk State University, monika_rainbow@mail.ru
Voronova Olga Sergeevna, Postgraduate student, Ulyanovsk State University, monika_rainbow@mail.ru
Gening Snezhanna Olegovna, Student, trainee-researcher, laboratory of molecular and cell biology, Research Technological Institute, Ulyanovsk State University, Naum-53@yandex.ru
Kostishko Boris Borisovich, Student, laboratory assistant, laboratory of scanning probe microscopy, Research Technological Institute, Ulyanovsk State University, Naum-53@yandex.ru

 61:577.3+535.3:612.128

Тhe authors have studied the effect of femtosecond laser radiation on human neutrophils in vitro. For this purpose the researchers have cytochemically determined the operating parameters of an aerobic bactericidal system and phagocytic activity. Besides that the topology and rigidity of a blood neutrophils membrane under the action of different doses of femtosecond laser radiation was also investigated. Rigidity of the neutrophils membrane changes and the number of cells producing reactive oxygen species increases under the influence of the femtosecond laser (spontaneous nitroblue tetrazolium test).

neutrophils, femtosecond laser irradiation, atomic force microscopy.